A diagnostic biomarker for Mycobacterium tuberculosis (Mtb) that performs well in children is urgently needed. Our project focuses on improving methods of diagnosing tuberculosis (TB) from urine: we will compare the ability to detect the lipoarabinomannan protein (Determine TB LAM Ag, Abbott, MA) with and without a specimen processing step using the Nanotrap method (Ceres Technology, Manassas, VA) which uses nanoparticle technology to concentrate proteins of interest and filter out interfering substances from urine. We anticipate that the Nanotrap method will augment the detection of LAM in urine samples from children diagnosed with TB.
Nanoparticle capture of urinary lipoarabinomannan for diagnosing childhood tuberculosis
Tania Thomas MD, MPH. Assistant Professor School of Medicine
Collaborator: Najeeha Iqbal, PhD. Rank: Assistant Professor Aga Khan University, Department of Paediatrics and Child Health, Pakistan